ABSTRACT
Objective: To study the callus induction and the content of active components magnolol and honokiol from Magnolia officinalis subsp. biloba and to find a new way to obtain medicinal ingredients in order to supplement the medicinal resources. Methods: Different vegetative organs from different provenances were adopted as explants, and different kinds of plant regulators with different concentration were used to induce the callus. The precursor compounds, L-phenylalanine and D, L, β-phenylalanine, were added into the subculture medium to induce the synthesis of callus; HPLC method was used to detect the total phenolic content of magnolol and honokiol in callus obtained through different culturing ways. Results: The optimal callus induction medium was: B5 + 6-BA 2.0 mg/L + 2, 4-D 1.5 mg/L; The total phenolic content of callus induced from different provenances had a significant difference in the range of 0.004%-0.228%; There was difference of total phenolic content in different regetative organs as explants, the callus cultured from young stems had the highest magnolol and honokiol content of 0.25%; Adding the precursor compound D, L, β-phenylalanine into the subculture medium could effectively improve the total phenolic content of callus by 8-10 times. Conclusion: There is little content of magnolol and honokiol in the callus of M. officinalis subsp. biloba, and the synthesis of total phenolic contents are significantly different in the callus from different provenances and different organs. D, L, β-phenylalanine could effectively improve the synthesis of total phenolic content in callus, and this research has the important significance for sustainable utilization of resources of M. officinalis subsp. biloba.
ABSTRACT
Objective: To establish a rapid propagation system of Magnolia officinalis subsp. biloba. Methods: Using peeling seeds of M. officinalis subsp. biloba as the initial explants, different media and various combinations of plant growth regulators (6-BA, NAA, IBA, and IAA) on the seeds germination, the seedlings subculture, and rooting culture were studied by single factor test and orthogonal test. Results: The best culture medium of seeds germination was B5 + 6-BA 0.5 mg/L + NAA 0.1 mg/L, and the budding percentage was 87.0%; The effective medium for cluster buds-inducing and subculture was MS + 6-BA 2.5 mg/L + NAA 0.5 mg/L, and the propagation coefficient was 6.2; The best rooting medium was 1/2 MS + NAA 0.5 mg/L, and the rooting rate was over 84% after 30 d. Conclusion: Rapid propagation technique system is established in order to lay technique foundation for the industrial production of M. officinalis subsp. biloba.
ABSTRACT
Objective: Huperzia serrata, whose growth is limited by high temperature, is a rare medicinal plant with the treatment function for Alzheimer's disease (AD). To research the effect of high temperature on the structure and function of cell membrane and chloroplast, and to provide the evidence for production practices. Methods: H. serrata was processed at 25, 30, 35, and 40°C, respectively, then the content changes of malondial dehyde (MDA) and conductivity rate, and the content changes of total chlorophyll, chlorophyll a, chlorophyll b, and chlorophyll a/b values were measured. The changes of the chloroplast ultra microstructure were observed under the transmission electron microscope (TEM). Results: The changes of MDA and conductivity rate in the process at 35 and 40°C were significantly higher than those of the control group; After processed at 40°C for 4 d, the total chlorophyll was decreased significantly, and became the lowest on the day 6, just was 58% compared to the control group; the change trends to the contents of chlorophyll a, chlorophyll b, and total chlorophyll were similar; TEM observation revealed that after processed at 35°C for 4 d, the chloroplast structure appeared deformation, and after processed at 40°C for 4 d, the chloroplast structure subjected obvious destruction capsule fuzzy, fracture in different degrees, thylakoid in disorder, matrix lamellar irregular, and so on. Conclusion: According to the changes of physiological index, ultramicroscopic structure, and external morphology of chloroplast, the suitable temperature for H. serrata is 25-30°C, 40°C is the limited temperature, causing death after 4 d stress, and 35°C has obvious impact on the growth, long-time stress in 35°C could also cause plant deaths.